nucspot live 650 nuclear stain (Biotium)

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Biotium nucspot live 650 nuclear stain
Nucspot Live 650 Nuclear Stain, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucspot+live+650/NucSpot+Live+488+Nuclear+Stain/bio_rxiv__2025__06__06__657607-32-15-20
Average 93 stars, based on 52 article reviews

nucspot live 650 nuclear stain - by Bioz Stars, 2026-09

93/100 stars

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Staining:

Article Title: The Nucleus Bypasses Obstacles by Deforming Like a Drop with Surface Tension Mediated by Lamin A/C.
Article Snippet: Hoechst (875756-97-1, Sigma-Aldrich, St. Louis, MO) was used to stain DNA, and Alexa Fluor-488 phalloidin (A12379, ThermoFisher Scientific, Waltham, MA) was used to stain F-actin in fixed samples. .. NucSpot Live 650 (40 082, Biotium, San Francisco, CA) was used to stain DNA in live cells. .. PlasMem Bright Red (P505, Dojindo Molecular Technologies, Inc., Rockville, MD) was used to stain the plasma membrane.

Article Title: Cholesterol accumulation impairs HIF-1α-dependent immunometabolic reprogramming of LPS-stimulated macrophages by upregulating the NRF2 pathway
Article Snippet: Cells were stained with 0.05 mg/mL Filipin III (Sigma-Aldrich, Oakville, ON) in PBS/10% FBS for 2 h at room temperature. .. After washing three times with PBS, nuclei were stained for 30 min with NucSpot Live 650 (2× in DMSO, Biotium, Fremont, CA) and plasma membrane, with 20 μg/mL Alexa Fluor-Concanavalin A (Sigma-Aldrich, Oakville, ON). .. Coverslips were mounted with Dako fluorescent mounting media (Dako, Santa Clara, CA).

Article Title: The Nucleus Bypasses Obstacles by Deforming Like a Drop with Surface Tension Mediated by Lamin A/C
Article Snippet: Hoechst (875756‐97‐1, Sigma‐Aldrich, St. Louis, MO) was used to stain DNA, and Alexa Fluor‐488 phalloidin (A12379, ThermoFisher Scientific, Waltham, MA) was used to stain F‐actin in fixed samples. .. NucSpot Live 650 (40 082, Biotium, San Francisco, CA) was used to stain DNA in live cells. .. PlasMem Bright Red (P505, Dojindo Molecular Technologies, Inc., Rockville, MD) was used to stain the plasma membrane.

Imaging:

Article Title: Conditional fluorescent mouse translocation reporters for ERK1/2 and AKT signaling.
Article Snippet: Understanding how cells activate intracellular signaling pathways in response to external signals, such as growth factors, is a longstanding goal of cell and developmental biology.. Recently, live-cell signaling reporters have greatly expanded our understanding of signaling dynamics in response to wide-ranging stimuli and chemical or genetic perturbation, both ex vivo (cell lines) and in vivo (whole embryos or animals).. Among the many varieties of reporter systems, translocation reporters that change sub-cellular localization in response to pathway activation have received considerable attention for their ease of use compared to FRET systems and favorable response times compared to transcriptional reporters.

Incubation:

Article Title: Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2
Article Snippet: .. After one day of culture, cells were incubated with NucSpot Live 650 (1:1,000; Biotium), the fluorescent Ca 2+ indicator Fluo-4-AM (1:500; Invitrogen), and 20% pluronic acid F-127 (1:500; Thermo P3000MP) for 1 hr at 37 °C. .. Cells were kept in medium and imaging was performed at 488 nm excitation for Fluo-4-AM and 647 nm excitation for NucSpot and emitted fluorescence was recorded with an LSM 780 inverted confocal microscope (Zeiss).

Article Title: Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2
Article Snippet: After one day of culture, cells were incubated with fluorescent PS binding protein annexin V-Alexa Flour 488 (1:10; Thermo A13201) in annexin-binding buffer for 15 min at room temperature. .. Cells were then washed with annexin-binding buffer and additionally incubated with NucSpot Live 650 (1:1,000; Biotium) for 1 hr at 37 °C. .. Cells were imaged at 488 nm excitation and emitted fluorescence was recorded with an LSM 780 inverted confocal microscope (Zeiss).

Article Title: Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2
Article Snippet: .. After incubation for 4 - 24 hours, cells were labeled by adding the dyes MemGlow 488 (Cytoskeleton; 1:200), SPY555-Actin (Cytoskeleton; 1:1,000), and NucSpot Live 650 (Biotium; 1:1,000). .. Migrating GBM cells in the microchannels were imaged every 5 min on a LSM 780 (Zeiss) confocal microscope (heated stage, 37 °C, with 5% CO 2 ) for up to 17 hr.

Clinical Proteomics:

Membrane:

Labeling:

Article Title: Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2
Article Snippet: .. The cells were labeled with the dyes SPY555-Actin (Cytoskeleton; 1:1,000) and NucSpot Live 650 (Biotium; 1:1,000) for 1 hr. .. The media was replaced with fresh media containing MemGlow 488 (Cytoskeleton; 1:200) and cells were incubated for 20 min at 4 °C.

Article Title: Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2
Article Snippet: .. After incubation for 4 - 24 hours, cells were labeled by adding the dyes MemGlow 488 (Cytoskeleton; 1:200), SPY555-Actin (Cytoskeleton; 1:1,000), and NucSpot Live 650 (Biotium; 1:1,000). .. Migrating GBM cells in the microchannels were imaged every 5 min on a LSM 780 (Zeiss) confocal microscope (heated stage, 37 °C, with 5% CO 2 ) for up to 17 hr.

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